University of Basel
DBM Flow Cytometry Core Facility

Design a panel and find antibodies today at

https://app.fluorofinder.com/baselbdmflowcore
Logo: FluoroFinder

Fortessa 2 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

DAPI
Live/Dead Fix Blue
DyLight 350

530 / 30

505

Brilliant Ultraviolet 496
Laser

405

450 / 50

--

Alexa Fluor 405
Brilliant Violet 421
eFluor 450
Pacific Blue
V450
CF405M
DyLight 405
SuperBright 436
Vio Blue

525 / 50

505

Brilliant Violet 510
V500
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
Vio Green

610 / 20

600

Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

660 / 20

630

Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

710 / 50

690

Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

750

Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

505

Alexa Fluor 488
Brilliant Blue 515
FITC
GFP
Venus
YFP
ATTO 488
CF488A
CF503R
Cy2

710 / 50

685

Brilliant Blue 700
PerCP
PerCP-Cy5.5
PerCP-eFluor 710
PerCP-Vio700
Laser

561

586 / 15

570

dsRed
dTomato
PE
CF550R
CF568
CF570

610 / 20

600

mCherry
PE-Alexa 610
PE-CF594
Propidium Iodide
DyLight 594
ECD
PE-Dazzle 594
PE-Texas Red
PE-Vio615

670 / 30

635

PE-Alexa 647
PE-Cy5
7-AAD

710 / 50

685

PE-Cy5.5

780 / 60

750

PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

640

670 / 14

--

Alexa Fluor 647
APC
Cy5
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cyanine 5 SE
DyLight 633
DyLight 650

730 / 45

690

Alexa Fluor 680
Alexa Fluor 700
APC-Alexa 700
APC-Cy5.5
APC-R700
DyLight 680

780 / 60

750

Alexa Fluor 750
APC-Alexa 750
APC-Cy7
APC-H7
Zombie NIR
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.