University of Antwerp
Antwerp Laboratory of Experimental Hematology

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FACSCanto II Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

488

530 / 30

502

 Alexa Fluor 488
Brilliant Blue 515
FITC
ATTO 488
CF488A
CF503R
Cy2
DyLight 488
Fluorescein
NL 493

585 / 42

556

 PE

695 / 40

655

 Brilliant Blue 700
PE-Cy5
PerCP
PerCP-Cy5.5
PerCP-eFluor 710
PerCP-Vio700

780 / 60

735

 PE-Cy7
PE-Vio770
PE-Alexa 750
Laser

633

660 / 20

--

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
NL 637

780 / 60

735

 APC-Cy7
APC-H7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.