Characterization of antigen-specific human T-cells
|Laurie Lamoreaux, Richard A. Koup, Mario Roederer Cytometry PART A, Volume 81A, Issue 5, 363-363 (2012)|
PURPOSE: The panels described in this article are designed to characterize the immunological response of human T-cells to vaccination by measuring the frequency, phenotype, and function of CD4 and CD8 T-cells. Subsequent qualification of the panel allows for comparison of intra- and inter-laboratory outcomes between different vaccine trials such that those vaccine formulations that reveal a possible correlate of protection against infection may be moved forward in the regulatory process. Although the panel was developed for batch analysis of cryopreserved PBMC samples, the assay may also be performed with fresh cells (Table 1).
CELL TYPE: Cryopreserved or Fresh Peripheral Blood Mononuclear Cells
MACHINE: BD LSR2
|Dead cells||Human||N/A||Live/Dead Fix Violet||viability|
|ccr7||Human||150503||Alexa Fluor 680||memory/differentiation|