PURPOSE: Cells undergoing myelopoiesis in both bone marrow and the mature myeloid compartments in the blood represent a broad range of phenotypes in different stages of maturation and activation. Neoplasms that arise from this lineage are often heterogeneous and frequently display unique characteristics and outcomes depending on the originating subset or degree of differentiation 1, 2. This is particularly true for myeloid neoplasms (MN) which rely on multiple, separate flow cytometry panels for differential diagnosis 3, 4. While standard practice, this approach has inherent disadvantages which include the inability to observe co‐expression patterns between markers contained in separate panels and increased time and labor associated with multiple flow cytometry assays. Use of a single, high‐order panel that is able to differentiate multiple hematopoietic lineages, or distinguish different MNs would aid this effort and provide the opportunity to further characterize disease subsets. This panel was designed to detect cell surface markers associated with hematopoiesis with a special emphasis on the progression of myelopoiesis from hematopoietic progenitors. Because the study of hematopoietic disease requires a broad combination of early hematopoietic and mature myeloid markers 5, this panel is also appropriate for the general analysis of myelopoiesis, granulopoiesis, erythropoiesis, and megakaryocytopoiesis, in any human cell source that contains mature myeloid cells, myeloid progenitors, or hematopoietic progenitors (Table 1).

CELL TYPE: Any source containing human myeloid cells

MACHINE: BD LSR2