< OMIPS Directory
OMIP-004

In-depth characterization of human T regulatory cells

Angelique Biancotto, Pradeep K. Dagur, J. Chris Fuchs, Marc Langweiler, J. Philip McCoy Jr Cytometry PART A, Volume 81A, Issue 1, 15-16 (2012)

PURPOSE: The present panel was constructed for the in-depth characterization of human T regulatory cells in both health and disease. The panel works well in both fresh and cryopreserved PBMCs (Table 1). The panel has also been tested on ACK-lysed peripheral blood. No other types of tissues have been tested.

CELL TYPE: Fresh or cryopreserved PBMCs, ACK-lysed blood

MACHINE: BD LSR Fortessa

Marker Target Clone Fluorochrome Purpose
Dead cells Human Live/Dead Fix Aqua Viability
CD45 Human Qdot 800 leukocyte
CD3 Human SK7 APC-Cy7 T cell
CD4 Human RPA-T4 V450 T helper subset
CD8 Human 3B5 Qdot 605 T suppressor subset
CD25 Human M-A251 PE-Cy7 Treg characterization
CD27 Human CLB-27/1 Qdot 655 Treg characterization
CD38 Human HIT2 PerCP-Cy5.5 Treg characterization
CD39 Human A1 Alexa Fluor 488 Treg supression
CD45RA Human PE-Texas Red naïve T cell
CD103 Human LF61 PE-Cy5 naïve T cell
CD127 Human hIL-7R-M21 Alexa Fluor 647 Treg characterization
ccr7 Human 150503 Alexa Fluor 700 Treg characterization
HLA-DR Human PE-Cy5.5 Treg characterization
FoxP3 Human PCH101 PE Treg marker