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OMIP-039

Detection and analysis of human adaptive NKG2C+ natural killer cells

Quirin Hammer, Chiara Romagnani Cytometry PART A, Volume 91, Issue 10, 997–1000 (2017)

PURPOSE: The present panel was optimized for the detection of adaptive natural killer (NK)‐cell populations in healthy human donors and offers versatility to investigate their biology including receptor usage, activation requirements, or signaling adaptor and transcription factor expression. It was established on cryopreserved PBMC and yields similar results with freshly isolated PBMC. Additional tissue samples have not been tested (Table 1).

CELL TYPE: Cryopreserved and freshly isolated PBMC

MACHINE: BD LSR Fortessa

Marker Target Clone Fluorochrome Purpose
Dead cells Human Zombie Aqua viability
CD14 Human M5E2 Brilliant Violet 510 exclusion
CD19 Human HIB19 Brilliant Violet 510 exclusion
CD3 Human UCHT1 PE-Cy5 exclusion
CD56 Human HCD56 PE-Dazzle 594 NK cells
CD159c Human REA205 PE Adaptive NK
CD159a (NKG2a) Human REA110 PE-Vio770 Conventional NK
CD337 Human AF29-4D12 eFluor 450 Conventional NK
CD328 Human REA214 APC-Vio770 Conventional NK
CD85j Human HP-F1 APC Adaptive NK
CD57 Human TBO1 Purified Adaptive NK
IgM Human RMM-1 Brilliant Violet 605 secondary reagent for CD57
CD2 Human RPA-2.10 PerCP-Cy5.5 Adaptive NK
CD7 Human M-T701 Brilliant Violet 786 Conventional NK