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OMIP-038

Innate immune assessment with a 14 color flow cytometry panel

Kinga K. Smolen, Bing Cai, Tobias R. Kollmann Cytometry PART A, Volume 91, Issue 10, 966–968 (2017)

PURPOSE: The purpose of the panel presented here is to assess the innate immune response in whole blood after Toll‐like receptor (TLR) ligand or cytokine stimulation, at the single‐cell level. In addition to the identification of many of the innate and innate‐adaptive interface immune cell subsets, this panel also enables the detection of key intracellular cytokines involved in the immune response.

CELL TYPE: Whole Blood

MACHINE: BD LSR2

Marker Target Clone Fluorochrome Purpose
Dead cells Human eFluor 780 Fix Viability Live/Dead
TNF alpha Human Mab11 Alexa Fluor 700 Function
CD11c Human S-HCL-3 APC cDC
CD123 Human 6H6 PE-Cy7 pDC (plasmacytoid DC)
CD3 Human UCHT1 PE-CF594 T lineage
IFN-alpha Human 7N4-1 PE Function
IL-6 Human MQ2-13A5 PerCP-eFluor 710 Function
TCR gamma/delta Human B1.1 FITC ?d T cell
CD66 Human ASL-32 Biotin Neutrophil
Streptavidin Human Brilliant Violet 786 secondary reagent to CD66
IFN-gamma Human 4S.B3 Brilliant Violet 711 Function
CD16 Human 3G8 Brilliant Violet 650 NK, NKT, monocyte
HLA-DR Human LN3 eFluor 605NC B cell, monocyte
CD14 Human M5E2 V500 Monocytes
IL-12 Human C8.6 eFluor 450 Function