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OMIP-030

Characterization of human T cell subsets via surface markers

Gerhard Wingender, Mitchell Kronenberg Cytometry PART A, Volume 87, Issue 12, 1067–1069 (2015)

PURPOSE: The present panel was optimized to quantify the relative frequency of the majority of the major T cell subsets currently described within human peripheral blood mononuclear cells (PBMCs) via the use of surface markers (Table 1). This includes all CD4+ T subsets that received a T helper—nomenclature to date and Tregs. Furthermore, a surrogate staining strategy for the identification of mNKT/MAIT cells without the need of Vα7.2 is proposed. The panel has been validated for fresh and cryopreserved PBMCs. Other tissue types have not been tested.

CELL TYPE: Fresh and cryopreserved PBMC

MACHINE: BD LSR Fortessa

Marker Target Clone Fluorochrome Purpose