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OMIP-027

Functional analysis of human natural killer cells

Margaret C. Costanzo, Matthew Creegan, Kerri G. Lal, Micheal A. Eller Cytometry PART A, Volume 87, Issue 9, 803-805 (2015)

PURPOSE: The current panel was developed to characterize the function of human natural killer (NK) cells from cryopreserved peripheral blood mononuclear cells (PBMC). The application of this panel is to identify changes in bulk NK cells and NK cell subsets with regard to receptor expression, and function in the setting of acute human immunodeficiency virus (HIV-1) infection. However, this panel may be applied to a wide variety of disease states and normal conditions to characterize human NK cells (Table 1). The performance of this panel was optimized using frozen PBMC from HIV-infected and uninfected individuals. The panel is being used to evaluate NK cell responses in individuals with acute HIV-1 infection as well as normal healthy individuals participating in HIV vaccine clinical trials.

CELL TYPE: Cryopreserved PBMC

MACHINE: BD LSR2

Marker Target Clone Fluorochrome Purpose