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Phenotypic characterization of human γδ T-cells by multicolor flow cytometry

Kilian Wistuba-Hamprecht, Graham Pawelec, Evelyna Derhovanessian Cytometry PART A, Volume 85, Issue 6, 522-524 (2013)

PURPOSE: This panel was composed and optimized to investigate the differentiation stages of human γδ T-cells in cryopreserved peripheral blood mononuclear cells (PBMC) from healthy individuals (Tables 1 and 2). As the majority of pan-γδ T-cell antibodies available commercially proved to be inappropriate for detecting all γδ T-cell populations in combination with other markers, this panel provides an essential tool for the analysis of different subsets of human γδ T-cells by flow cytometry. The panel works very well with cryopreserved PBMC. Other tissues have not been tested.



Marker Target Clone Fluorochrome Purpose
Dead cells Human EMA viability
CD3 Human UCHT1 Alexa Fluor 700 Lineage
CD4 Human OKT4 PE-Cy7 CD4 T cell
CD8 Human SK1 APC-H7 CD8 T cell
CD16 Human 3G8 Brilliant Violet 711 ?dT activation
CD27 Human APC differentiation
CD28 Human CD28.2 PE differentiation
CD45RA Human Pacific Blue differentiation
TCR gamma/delta Human 11F2 Purified ?dT cell
IgG Human N/A Pacific Orange secondary reagent to ?d-TCR (clone qqF2)
TCR V delta 1 Human TS8.2 FITC ?dT cell subset
TCR V delta 2 Human B6 PerCP ?dT cell subset