< OMIPS Directory
OMIP-019

Quantification of human γδT-cells, iNKT-cells, and hematopoietic precursors

Yolanda D. Mahnke, Margaret H. Beddall, Mario Roederer Cytometry PART A, Volume 83A, Issue 8, 676-678 (2013)

PURPOSE: The present panel was optimized to quantify the relative frequencies of γδT-cells, invariant natural killer T-cells (iNKT-cells), and hematopoietic precursors in peripheral blood mononuclear cells (PBMC) from healthy individuals (Table 1). It works well with cryopreserved PBMC and we have observed similar results with fresh specimens. Other tissue types have not been tested.

CELL TYPE: PBMC

MACHINE: BD LSR2

Marker Target Clone Fluorochrome Purpose
Dead cells Human Live/Dead Fix Aqua viability
CD28 Human CD28.2 PE-Cy5 Surface
CCR5 Human 2D7/CCR5 APC-Cy7 phenotyping
CD4 Human OKT4 Brilliant Violet 605 phenotyping
CD34 Human HI100 Brilliant Violet 421 Hematopoietic stem cell
TCR V gamma 9 Human B3 APC ?dT cell
TCR V delta 1 Human TS8.2 FITC ?dT cell
CD45RA Human MEM-56 PE-Cy5.5 phenotyping
CD1d Human PE iNKT
CD3 Human OKT3 Brilliant Violet 785 lineage
ccr7 Human 150503 Alexa Fluor 680 phenotyping
CD8 Human RPA-T8 Qdot 585 phenotyping
CD27 Human Qdot 655 phenotyping
TCR V delta 2 Human B6 Alexa Fluor 594 ?dT cell