< OMIPS Directory
OMIP-017

Human CD4+ helper T-cell subsets including follicular helper cells

Yolanda D. Mahnke, Margaret H. Beddall, Mario Roederer Cytometry PART A, Volume 83A, Issue 5, 439-440 (2013)

PURPOSE: This panel was optimized to measure the relative frequencies of CD4+ T-helper cell subsets and follicular helper T-cells in peripheral blood mononuclear cells (PBMC) from healthy individuals (Table 1). It works well with cryopreserved PBMC and we have observed similar result with fresh specimens. Other tissue types have not been tested.

CELL TYPE: PBMC

MACHINE: BD LSR2

Marker Target Clone Fluorochrome Purpose
Dead cells Human Live/Dead Fix Aqua viability
CD194 Human PE-Cy7 Th subset (chemokine leve)
CCR6 Human G034E3 Brilliant Violet 605 Th subset (chemokine leve)
CCR10 Human 6588-5 PE Th subset (chemokine leve)
CD183 Human 1C6/CXCR3 PE-Cy5 Th subset (chemokine leve)
CD185 Human RF8B2 Alexa Fluor 647 Tfh
PD-1 Human EH12.2H7 Brilliant Violet 421 exploratory
CD3 Human UCHT1 Alexa Fluor 594 lineage
CD4 Human RPA-T4 Qdot 800 lineage
CD8 Human RPA-T8 Qdot 585 lineage
CD45RA Human 5H9 Qdot 655 differentiation
CD161 Human DX12 FITC exploratory
ccr7 Human 150503 Alexa Fluor 680 differentiation