Human regulatory and activated T-cells without intracellular staining
|Yolanda D. Mahnke, Margaret H. Beddall, Mario Roederer Cytometry PART A, Volume 83A, Issue 2, 179-181 (2013)|
PURPOSE: The present panel was optimized to investigate the frequency and phenotype of regulatory T-cells (Treg), as well as the activation status of CD4+ and CD8+ T-cells in peripheral blood mononuclear cells (PBMC) from healthy individuals, without the use of intracellular staining (i.e., excluding the use of the canonical Treg marker, FoxP3). The panel has been developed using cryopreserved PBMC and we have observed similar results with fresh specimens. Other tissue types have not been tested.
CELL TYPE: PBMC
MACHINE: BD LSR2
|Dead cells||Human||Live/Dead Fix Aqua||viability|
|PD-1||Human||EH12.2H7||Brilliant Violet 421||activation/differentiation|
|HLA-DR||Human||G46-6||Alexa Fluor 680||activation/differentiation|