< OMIPS Directory
OMIP-013

Differentiation of human T-cells

Yolanda D. Mahnke, Margaret H. Beddall, Mario Roederer Cytometry PART A, Volume 81A, Issue 11, 935-936 (2012)

PURPOSE: The present panel was optimized to investigate the differentiation status of CD4+ and CD8+ T-cells in peripheral blood mononuclear cells (PBMC) fromhealthy individuals. It works well with cryopreserved PBMC and we have observed similar results with fresh specimens. Other tissue types have not been tested.

CELL TYPE: PBMC

MACHINE: BD LSR2

Marker Target Clone Fluorochrome Purpose
Dead cells Human Live/Dead Fix Aqua viability
CD127 Human A019D5 Brilliant Violet 421 memory/differentiation for TSCM
CD95 Human Dx2 PE memory/differentiation for RTE and TSCM
CD244 Human C1.7 PE-Cy5.5 memory/differentiation (exploratory)
CD31 Human WM59 PE-Cy7 memory/differentiation for RTE and TSCM
CD27 Human O323 FITC memory/differentiation
CD28 Human CD28.2 PE-Cy5 memory/differentiation for TSCM
CD45RA Human HI100 APC memory/differentiation
CD3 Human SK7 APC-H7 T-cell subset
CD8 Human RPA-T8 Qdot 585 T-cell subset
CD4 Human OKT4 Qdot 605 T-cell subset
CD57 Human NK-1 Qdot 705 memory/differentiation for TSCM
ccr7 Human 150503 Alexa Fluor 680 memory/differentiation