< OMIPS Directory
OMIP-078

A 31-parameter panel for comprehensive immunophenotyping of multiple immune cells in human peripheral blood mononuclear cells

Takuto Nogimori, Yuko Sugawara, Masaya Higashiguchi, Hirotomo Murakami, Hirofumi Akita, Shokichi Takahama, Satoshi Tanaka, Takuya Yamamoto Cytometry PART A, Volume 99, Issue 9, 893-898 (2021)

PURPOSE: This 31-parameter panel was developed for simultaneously measuring multiple immune cell populations including T cells, B cells, natural killer cells, dendritic cells, monocytes, and hematopoietic progenitor cells in human peripheral blood mononuclear cells. This panel enables the capture of individual immune dynamics and assessments of single-cell changes in the immune system that are associated with aging and diseases. This panel includes markers to separate the differentiation status of each cell population and might be applicable to studies of infectious and autoimmune diseases, as patient samples are usually limited in volume and require an analysis system that provides a relatively large amount of information.

CELL TYPE: Fresh or cryopreserved PBMCs from healthy donors.

MACHINE: BD FACSymphony A5

Marker Target Clone Fluorochrome Purpose
FVS700 All Species FVS700 Viability
CD57 Human NK-1 FITC
CD127 Human HIL-7R-M21 BB630-P2
CD38 Human HIT2 BB660-P2
CD34 Human 8G12 BB700
CD1c Human F10/21A3 BB755-P
IgM Human (Negative) G20-127 BB790-P
CD133 Human W6B3C1 PE
CD11c Human PE-CF594
CD56 Human B159 PE-Cy5
CD4 Human SK3 PE-Cy5.5
CD27 Human M-T271 PE-Cy7
IgD Human IA6-2 APC
CD3 Human SP34-2 APC-Cy7
CD303 Human V24-785 Brilliant Violet 421
CD123 Human 7G3 Brilliant Violet 480
CD45RO Human UCHL1 Brilliant Violet 570
CD138 Human MI15 Brilliant Violet 605
CD16 Human 3G8 Brilliant Violet 650
CD19 Human SJ25C1 Brilliant Violet 711
CD24 Human ML5 Brilliant Violet 750
PD-1 Human EH12.1 Brilliant Violet 786
CD11b Human ICRF44 BUV395
CD45 Human HI30 BUV496
CD8 Human RPA-T8 BUV563
CD141 Human 1A4 BUV615
HLA-DR Human G46-6 BUV661
IgG Human G18-145 BUV737
CD14 Human M5E2 BUV805