< OMIPS Directory
OMIP-077

Definition of all principal human leukocyte populations using a broadly applicable 14-color panel

Maximilian Boesch, Martina Sykora, Silvia Gasteiger, Florent Baty, Martin H. Brutsche, Sieghart Sopper TBD

PURPOSE: The present optimized multicolor immunofluorescence panel (OMIP) provides for the detection and in-depth analysis of all major leukocyte subsets in human whole blood (WB) using a single flow cytometry (FCM) panel with just 14 colors. The panel offers an easy, fast and reproducible way of comprehensive immune cell profiling for various purposes including translational research and companion immune monitoring of clinical trials.

CELL TYPE: Leukocytes from human WB (anticoagulated, erythrocyte-depleted)

MACHINE: BD LSR Fortessa

Marker Target Clone Fluorochrome Purpose
CD1c Human AD5-8E7 PE CD1c+ mDCs, MZB cells
CD3 Human OKT3 Brilliant Violet 605 T cells
CD14 Human M?P9 (also known as M?P-9) Brilliant Violet 711 Monocytes (c), intermediate monocytes, monocytes (nc)
CD15 Human HI98 PerCP-Cy5.5 Eosinophils
CD16 Human eBioCB16 (CB16) APC-eFluor 780 Neutrophils, intermediate monocytes, monocytes (nc)
CD19 Human HIB19 APC-R700 B cells, plasma cells
CD34 Human 8G12 (also known as HPCA2) FITC Progenitor cells
CD38 Human HIT2 Brilliant Violet 421 Plasma cells, basophils
CD45 Human HI30 Brilliant Violet 480 Pan-leukocytes
CD56 Human NCAM16.2 (also known as NCAM 16) PE-Cy7 NK cells
CD123 Human 7G3 Brilliant Violet 650 Basophils, pDCs
CD141 Human AD5-14H12 APC CD141+ mDCs
CD193 Human 5E8 (also known as 5E8-G9-B4) PE-CF594 Eosinophils, basophils
HLA-DR Human G46-6 Brilliant Violet 786 Neutrophils, monocytes (c), intermediate monocytes, monocytes (nc), B cells, plasma cells, CD1c+ mDCs, CD141+ mDCs