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High-dimensional immunophenotyping of murine T-cell, B-cell, and antibody secreting cell subsets

Kyle T. Mincham, Jacob D. Young, Deborah H. Strickland Cytometry PART A, Volume 99, Issue 9, 888-892 (2021)

PURPOSE: This 19-parameter, 18-color flow cytometry panel was designed and optimized to enable the comprehensive and simultaneous immunophenotyping of distinct T-cell, B-cell, and antibody secreting cell (ASC) subsets within murine tissues.

CELL TYPE: Murine tissues containing lymphocyte populations


Marker Target Clone Fluorochrome Purpose
CD273 Mouse TY25 BUV395 Memory B-cells
IgD Mouse AMS 9.1 BUV496 Activated/memory B-cells
CD44 Mouse IM7 BUV737 T-cell subsets
CD278 Mouse 7E.17G9 Brilliant Violet 421 T Follicular helper/Treg
PD-1 Mouse J43 Brilliant Violet 480 T Follicular helper cells
FVS575V All Species FVS575V Viable cells
CD80 Mouse 16-10A1 Brilliant Violet 650 Activated B-cells
IgM Mouse R6-60.2 Brilliant Violet 711 B-cell/ASC subsets
CD4 Mouse RM4-5 Brilliant Violet 786 CD4+ T-cells
CD19 Mouse 1D3 Brilliant Violet 786 B-cell subsets
CD138 Mouse 281-2 BB515 Plasmablasts/Plasma cells
TCR beta Mouse H57-597 BB700 Pan T-cells
FoxP3 Mouse FJK-16s PE Regulatory T-cells
CD45R Mouse RA3-6B2 PE-CF594 B-cell subsets
CD25 Mouse PC61 PE-Cy5 Activated T-cells
CD185 Mouse 2G8 PE-Cy7 T Follicular helper cells
MHC Class II (I-A/I-E) Mouse M5/114.15.2 (also known as M5/114) Alexa Fluor 647 B-cell subsets
CD62L Mouse Mel-14 APC-R700 T-cell subsets
CD45 Mouse 30-F11 APC-Cy7 Pan leukocyte