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A 22-color panel for the measurement of antigen-specific T-cell responses in human and nonhuman primates

Takuto Nogimori, Eiko Moriishi, Mami Ikeda, Shokichi Takahama, Takuya Yamamoto Cytometry PART A, Volume 99, Issue 9, 884-887 (2021)

PURPOSE: We aimed to design a multicolor panel to detect antigen-specific CD4+ T cells in non-human 4 primate models (NHPs), for vaccine-related and infectious disease-related studies...



Marker Target Clone Fluorochrome Purpose
Live/Dead Fix Blue All Species Live/Dead Fix Blue Viability
CD3 Human SP34-2 BUV615 Surface Staining
CD4 Human S3.5 PE-Cy5.5 Surface Staining
CD8 Human RPA-T8 BUV563 Surface Staining
CD45RO Human UCHL1 BUV805 Surface Staining
CD27 Human 1A4CD27 PE-Cy5 Surface Staining
CD28 Human CD28.2 BUV805 Surface Staining
CD95 Human DX2 PE-Cy5 Surface Staining
PD-1 Human EH12.2H7 Brilliant Violet 750 Surface Staining
CD278 Human C398.4A BUV395 Surface Staining
CD183 Human G025H7 PE Surface 37˚C Staining
CD185 Human MU5UBEE PE-eFluor 610 Intracellular Staining
CD194 Human L291H4 Brilliant Violet 510 Surface 37˚C Staining
CCR6 Human G034E3 APC-Fire 750 Surface 37˚C Staining
CD40L Human TRAP1 FITC Intracellular Staining
IL-2 Human MQ1-17H12 BUV737 Intracellular Staining
IL-4 Human 8D4-8 PE-Cy7 Intracellular Staining
IL-13 Human JES10-5A2 Brilliant Violet 421 Intracellular Staining
IL-17A Human BL168 Brilliant Violet 605 Intracellular Staining
IL-21 Human 3A3-N2.1 (also known as 3A3-N2) Alexa Fluor 647 Intracellular Staining
MIP-1 beta Human D21-1351 Alexa Fluor 700 Intracellular Staining
IFN-gamma Human 4S.B3 Brilliant Violet 786 Intracellular Staining
TNF alpha Human MAb11 Brilliant Violet 650 Intracellular Staining
CD107a Human H4A3 Brilliant Violet 711 Co-culture