< OMIPS Directory
OMIP-074

Phenotypic analysis of IgG and IgA subclasses on human B cells

Leonard Nettey, Reid Ballard, Thomas Liechti, Rosemarie D. Mason Cytometry PART A, Volume 99, Issue 9, 880-883 (2021)

PURPOSE: This panel was designed and optimized to characterize the phenotypic diversity of circulating human memory B cells with an emphasis on discriminating IgA and IgG subclasses.

CELL TYPE: Fresh or cryopreserved PBMC

MACHINE: FACSymphony A5

Marker Target Clone Fluorochrome Purpose
CD19 Human HIB19 PE-Dazzle 594 Lineage
CD20 Human 2H7 Alexa Fluor 700 B-Cell Subset
CD27 Human O323 Brilliant Violet 650
CD21 Human B-ly4 Brilliant Violet 605
IgA Human DyLight 405 Immunoglobulin Isotype
IgD Human PE-Cy5.5
IgE Human MHE-18 APC-Fire 750
IgG Human G18-145 PE-Cy5
IgM Human MHM-88 Brilliant Violet 570
IgA1 Human B3506B4 PerCP-Cy5.5 Immunoglobulin subclass
IgA2 Human IS11-21E11 PE-Vio770
IgG1 Human HP6001 PE
IgG2 Human HP6002 Biotin
Biotin Human Brilliant Violet 750
IgG3 Human HP6050 Alexa Fluor 488
IgG4 Human APC
Ig Kappa Light Chain Human G20-193 Brilliant Violet 711 Immunoglobulin light chain
Ig Lambda Light Chain Human JDC-12 Brilliant Violet 786
Live/Dead Fix Aqua Human Live/Dead Fix Aqua Viability