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A new 10-color monoclonal antibody panel for polychromatic immunophenotyping of small hematopoietic cell samples

Frank W. M. B. Preijers, Erik Huys, Bijan Moshaver Cytometry PART A, Volume 81A, Issue 6, 453-455 (2012)

PURPOSE: The 10-color panel consisting of 15 monoclonal antibodies (mAbs) is developed to detect leukemia and lymphoma cells in small cell samples [hypoplastic bone marrow (BM), fine needle aspirates, or cerebral spinal fluids (CSFs)]. MAbs conjugates were selected to identify populations of distinct cell lineages and to determine stages of differentiation based on specific antigen expression patterns. As such, conjugates containing the same fluorochrome could be combined. This panel is tested on peripheral blood (PB), BM, and CSF and provides a strong improvement of diagnostic potential.

CELL TYPE: Fresh Cerebral Spinal Fluids, Bone Marrow, Peripheral Blood

MACHINE: Beckman Coulter Navios

Marker Target Clone Fluorochrome Purpose
CD34 Human 581 FITC lymphoid and meyloid progenitors
Ig Lambda Light Chain Human FITC secondary reagent to CD34
CD7 Human 8H8.1 PE T cell
Ig Lambda Light Chain Human PE secondary reagent to CD7
CD10 Human ALB1 ECD B cell precursors
CD4 Human PE-Cy5.5 T cell
CD56 Human PE-Cy7 NK, NKT
CD117 Human PE-Cy7 lymphoid and meyloid progenitors
CD15 Human 80H5 Pacific Blue B
CD20 Human B9E9 Pacific Blue B
CD45 Human J33 Krome Orange lymphocyte
CD3 Human UCHT1 APC T cell
CD33 Human D3HL60.251 APC T cell
CD8 Human B9.11 APC-Alexa 700 T cell
CD19 Human J3-119 APC-Alexa 750 B