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Quality and phenotype of Ag-responsive human T-cells

Yolanda D. Mahnke, Mario Roederer Cytometry PART A, Volumn 77A, Issue 9, 819-820 (2010)

PURPOSE: The present panel was optimized for the evaluation of CD4+ and CD8+ T-cell responses to various HIV-1–derived peptide pools in peripheral blood mononuclear cells (PBMC) from HIV-1+ individuals with differences in clinical progression. It works well with cryopreserved PBMC, and we have observed similar results with fresh specimens. Other tissue types have not been tested.

CELL TYPE: Peripheral Blood Mononuclear Cells


Marker Target Clone Fluorochrome Purpose
Dead cells Human Live/Dead Fix Violet viability
CD4 Human M-T477 Qdot 605 T cell Lineage
IL-2 Human MQ1-17H12 Alexa Fluor 488 function
CD127 Human R34.34 PE memory/differentiation
CD28 Human CD28.2 PE-Cy5 memory/differentiation
IFN-gamma Human B27 APC function
CD3 Human SK7 APC-Cy7 T cell Lineage
CD14 Human M5E2 Pacific Blue exclusion
CD19 Human HIB19 Pacific Blue exclusion
CD45RO Human UCHL1 Qdot 545 memory/differentiation
CD8 Human RPA-T8 Qdot 585 T cell Lineage
PD-1 Human MIH4 Biotin memory/differentiation
Biotin Human Streptavidin Qdot 655 secondary reagent to PD-1
CD57 Human NK-1 Qdot 705 memory/differentiation
TNF alpha Human Mab11 Alexa Fluor 594 function
CD27 Human M-T271 PE-Cy7 memory/differentiation
ccr7 Human 150503 Alexa Fluor 680 memory/differentiation