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MITO-ID (Extracellular O2)

MITO-ID (Extracellular O2) (Ex-Max 379 nm/Em-Max 644 nm)

MITO-ID Extracellular O2 sensor from Enzo Life Sciences is an oxygen sensitive phosphorescent probe used to assess O2 consumption in cultured cells, organelles and tissue using a fluorescent microplate reader. It has an excitation max at 379 nm and an emission max at 644 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
379 nm
Emission maximum
644 nm
Optimal laser line
355
Optimal filter
630/69

MITO-ID (Extracellular O2) has an excitation maximum at 379 nm and an emission maximum at 644 nm, a Stokes shift of 265 nm. It is optimally excited by the 355 laser line.

Frequently asked questions

What is MITO-ID (Extracellular O2)?
MITO-ID Extracellular O2 sensor from Enzo Life Sciences is an oxygen sensitive phosphorescent probe used to assess O2 consumption in cultured cells, organelles and tissue using a fluorescent microplate reader. It has an excitation max at 379 nm and an emission max at 644 nm.
What laser excites MITO-ID (Extracellular O2)?
MITO-ID (Extracellular O2) is optimally excited by the 355 laser line. Its excitation maximum is 379 nm.
What filter should I use to detect MITO-ID (Extracellular O2)?
A 630/69 filter captures the peak of the MITO-ID (Extracellular O2) emission near 644 nm with minimal spillover.