Nucleolar-ID Green
Nucleolar-ID Green (Ex-Max 439 nm/Em-Max 477 nm)
Nucleolar-ID Green is a cell-permeant, fluorogenic nucleic acid stain used for the detection of nucleoli in living cells. It is excited at 440 nm and emits at 500nm. It can be used with the following analysis platforms: Fluorescence Microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 439 nm
- Emission maximum
- 477 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
Nucleolar-ID Green has an excitation maximum at 439 nm and an emission maximum at 477 nm, a Stokes shift of 38 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Nucleolar-ID Green?
- Nucleolar-ID Green is a cell-permeant, fluorogenic nucleic acid stain used for the detection of nucleoli in living cells. It is excited at 440 nm and emits at 500nm. It can be used with the following analysis platforms: Fluorescence Microscopy.
- What laser excites Nucleolar-ID Green?
- Nucleolar-ID Green is optimally excited by the 405 laser line. Its excitation maximum is 439 nm.
- What filter should I use to detect Nucleolar-ID Green?
- A 450/50 filter captures the peak of the Nucleolar-ID Green emission near 477 nm with minimal spillover.