eYFP
eYFP (Ex-Max 516 nm/Em-Max 528 nm)
eYFP is a fluroescent protein derived from Aequorea victoria.
Excitation & emission spectra
Key facts
- Excitation maximum
- 516 nm
- Emission maximum
- 528 nm
- Optimal filter
- 525/50
- Extinction coefficient
- 67000
- Quantum yield
- 0.67
- Molecular weight
- 27000
eYFP has an excitation maximum at 516 nm and an emission maximum at 528 nm, a Stokes shift of 12 nm. Its extinction coefficient is 67000 M⁻¹cm⁻¹.
Frequently asked questions
- What is eYFP?
- eYFP is a fluroescent protein derived from Aequorea victoria.
- What filter should I use to detect eYFP?
- A 525/50 filter captures the peak of the eYFP emission near 528 nm with minimal spillover.