Newport Green DCF
Newport Green DCF (Ex-Max 469 nm/Em-Max 530 nm)
Newport Green DCF Diacetate is a cell-permeant Zinc indicator. It has an excitation peak at 469 nm and an emission peak at 530 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 469 nm
- Emission maximum
- 530 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Extinction coefficient
- 70000
- Quantum yield
- 0.06
Newport Green DCF has an excitation maximum at 469 nm and an emission maximum at 530 nm, a Stokes shift of 61 nm. Its extinction coefficient is 70000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Newport Green DCF?
- Newport Green DCF Diacetate is a cell-permeant Zinc indicator. It has an excitation peak at 469 nm and an emission peak at 530 nm.
- What laser excites Newport Green DCF?
- Newport Green DCF is optimally excited by the 488 laser line. Its excitation maximum is 469 nm.
- What filter should I use to detect Newport Green DCF?
- A 525/50 filter captures the peak of the Newport Green DCF emission near 530 nm with minimal spillover.