Indo-Hi (Ca2+bound)
Indo-Hi (Ca2+bound) (Ex-Max 329 nm/Em-Max 401 nm)
Indo-1, AM is a ratiometric calcium indicator optimally excited by a 355nm UV laser or excitation source. After Indo-1, AM is loaded into live cells, it is hydrolyzed and available to bind cytoplasmic Ca2+. Probe bound to Ca2+ emit at ~400nm, while probe unbound to Ca2+ emits at ~475nm. Because of these unique spectral characteristics, it's the preferred calcium indicator for flow cytometry and is often frequently used in fluorescence live-cell microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 329 nm
- Emission maximum
- 401 nm
- Optimal laser line
- 355
- Optimal filter
- 379/28
- Commercial antibodies
- 1 from 1 supplier
Indo-Hi (Ca2+bound) has an excitation maximum at 329 nm and an emission maximum at 401 nm, a Stokes shift of 72 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is Indo-Hi (Ca2+bound)?
- Indo-1, AM is a ratiometric calcium indicator optimally excited by a 355nm UV laser or excitation source. After Indo-1, AM is loaded into live cells, it is hydrolyzed and available to bind cytoplasmic Ca2+. Probe bound to Ca2+ emit at ~400nm, while probe unbound to Ca2+ emits at ~475nm. Because of these unique spectral characteristics, it's the preferred calcium indicator for flow cytometry and is often frequently used in fluorescence live-cell microscopy.
- What laser excites Indo-Hi (Ca2+bound)?
- Indo-Hi (Ca2+bound) is optimally excited by the 355 laser line. Its excitation maximum is 329 nm.
- What filter should I use to detect Indo-Hi (Ca2+bound)?
- A 379/28 filter captures the peak of the Indo-Hi (Ca2+bound) emission near 401 nm with minimal spillover.
- How many antibodies are available conjugated to Indo-Hi (Ca2+bound)?
- FluoroFinder tracks 1 commercially available Indo-Hi (Ca2+bound) conjugates, searchable by target, clone, host and application.