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APC-Fire 750

APC-Fire™ 750 (Ex-Max 651 nm/Em-Max 788 nm)

APC-Fire™ 750 from Biolegend has an excitation peak at 651 nm and an emission peak at 788 nm and is most commonly used in flow cytometry. Spectrally its similar to APC-Cy7, APC-Alexa 750 and APC-H7

Excitation & emission spectra

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Key facts

Excitation maximum
651 nm
Emission maximum
788 nm
Optimal laser line
628-640
Optimal filter
780/60
Relative brightness
2 of 5
Commercial antibodies
1.3k from 2 suppliers

APC-Fire 750 has an excitation maximum at 651 nm and an emission maximum at 788 nm, a Stokes shift of 137 nm. It is optimally excited by the 628-640 laser line.

Frequently asked questions

What is APC-Fire 750?
APC-Fire™ 750 from Biolegend has an excitation peak at 651 nm and an emission peak at 788 nm and is most commonly used in flow cytometry. Spectrally its similar to APC-Cy7, APC-Alexa 750 and APC-H7
What laser excites APC-Fire 750?
APC-Fire 750 is optimally excited by the 628-640 laser line. Its excitation maximum is 651 nm.
What filter should I use to detect APC-Fire 750?
A 780/60 filter captures the peak of the APC-Fire 750 emission near 788 nm with minimal spillover.
How bright is APC-Fire 750?
APC-Fire 750 has a relative brightness of 2 out of 5.
How many antibodies are available conjugated to APC-Fire 750?
FluoroFinder tracks 1346 commercially available APC-Fire 750 conjugates, searchable by target, clone, host and application.