Hoechst (33258)
Hoechst (33258) (Ex-Max 352 nm/Em-Max 454 nm)
Hoechst (33258) is a cell-permeant, fluorogenic nucleic acid stain. It has an excitation peak at 352 nm and an emission peak at 454 nm. It is useful in counter-staining nuclei in both flow cytometry and microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 352 nm
- Emission maximum
- 454 nm
- Optimal laser line
- 355
- Optimal filter
- 450/50
- Extinction coefficient
- 46000
- Commercial antibodies
- 4 from 2 suppliers
Hoechst (33258) has an excitation maximum at 352 nm and an emission maximum at 454 nm, a Stokes shift of 102 nm. Its extinction coefficient is 46000 M⁻¹cm⁻¹. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is Hoechst (33258)?
- Hoechst (33258) is a cell-permeant, fluorogenic nucleic acid stain. It has an excitation peak at 352 nm and an emission peak at 454 nm. It is useful in counter-staining nuclei in both flow cytometry and microscopy.
- What laser excites Hoechst (33258)?
- Hoechst (33258) is optimally excited by the 355 laser line. Its excitation maximum is 352 nm.
- What filter should I use to detect Hoechst (33258)?
- A 450/50 filter captures the peak of the Hoechst (33258) emission near 454 nm with minimal spillover.
- How many antibodies are available conjugated to Hoechst (33258)?
- FluoroFinder tracks 4 commercially available Hoechst (33258) conjugates, searchable by target, clone, host and application.