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Krome Orange

Krome Orange (Ex-Max 398 nm/Em-Max 528 nm)

Krome Orange from Beckman Coulter is a fluorophore with an excitation peak at 398 nm and an emission peak at 528 nm. It can be used in flow cytometry.

Excitation & emission spectra

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Key facts

Excitation maximum
398 nm
Emission maximum
528 nm
Optimal laser line
405
Optimal filter
525/30
Relative brightness
1 of 5
Commercial antibodies
12 from 1 supplier

Krome Orange has an excitation maximum at 398 nm and an emission maximum at 528 nm, a Stokes shift of 130 nm. It is optimally excited by the 405 laser line.

Frequently asked questions

What is Krome Orange?
Krome Orange from Beckman Coulter is a fluorophore with an excitation peak at 398 nm and an emission peak at 528 nm. It can be used in flow cytometry.
What laser excites Krome Orange?
Krome Orange is optimally excited by the 405 laser line. Its excitation maximum is 398 nm.
What filter should I use to detect Krome Orange?
A 525/30 filter captures the peak of the Krome Orange emission near 528 nm with minimal spillover.
How bright is Krome Orange?
Krome Orange has a relative brightness of 1 out of 5.
How many antibodies are available conjugated to Krome Orange?
FluoroFinder tracks 12 commercially available Krome Orange conjugates, searchable by target, clone, host and application.