Brilliant Violet 480
Brilliant™ Violet 480 (Ex-Max 436 nm/Em-Max 478 nm)
Brilliant™ Violet 480 (BV480) is a green-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 525/40 bandpass filter. BV480 has an excitation peak at 436 nm and an emission peak at 478 nm, and provides an optimized alternative to BV510. Other dyes that are similar include StarBright Violet 475 (Bio-Rad) While BV480 can be detected on the same filter as BV510, its emmision profile reduces spillover into the BV605, BV650, and BV711 channels. Additionally BV480's excitation profile will reduce cross-laser excitation with the UV laser, resulting in less spillover into UV channels than BV510. These advantages make BV480 an optimal choice for flow cytometry panels using multiple reagents on Violet and/or UV lasers. This dye is part of the Brilliant™ Violet dye line. BV510 and BV480 are the only dyes in the Brilliant™ Violet dye family that are not tandem fluorophores based off the BV421 polymer core, but they have nearly the same maximum excitation.
Excitation & emission spectra
Key facts
- Excitation maximum
- 436 nm
- Emission maximum
- 478 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Relative brightness
- 2 of 5
- Extinction coefficient
- 1416000
- Quantum yield
- 0.81
- Commercial antibodies
- 1k from 3 suppliers
Brilliant Violet 480 has an excitation maximum at 436 nm and an emission maximum at 478 nm, a Stokes shift of 42 nm. Its extinction coefficient is 1416000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Brilliant Violet 480?
- Brilliant™ Violet 480 (BV480) is a green-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 525/40 bandpass filter. BV480 has an excitation peak at 436 nm and an emission peak at 478 nm, and provides an optimized alternative to BV510. Other dyes that are similar include StarBright Violet 475 (Bio-Rad) While BV480 can be detected on the same filter as BV510, its emmision profile reduces spillover into the BV605, BV650, and BV711 channels. Additionally BV480's excitation profile will reduce cross-laser excitation with the UV laser, resulting in less spillover into UV channels than BV510. These advantages make BV480 an optimal choice for flow cytometry panels using multiple reagents on Violet and/or UV lasers. This dye is part of the Brilliant™ Violet dye line. BV510 and BV480 are the only dyes in the Brilliant™ Violet dye family that are not tandem fluorophores based off the BV421 polymer core, but they have nearly the same maximum excitation.
- What laser excites Brilliant Violet 480?
- Brilliant Violet 480 is optimally excited by the 405 laser line. Its excitation maximum is 436 nm.
- What filter should I use to detect Brilliant Violet 480?
- A 450/50 filter captures the peak of the Brilliant Violet 480 emission near 478 nm with minimal spillover.
- How bright is Brilliant Violet 480?
- Brilliant Violet 480 has a relative brightness of 2 out of 5 (quantum yield 0.81).
- How many antibodies are available conjugated to Brilliant Violet 480?
- FluoroFinder tracks 1029 commercially available Brilliant Violet 480 conjugates, searchable by target, clone, host and application.