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BFP

BFP is a blue fluorescent protein derived from Aequorea victoria with an excitation peak at 374 nm and an emission peak at 443 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
374 nm
Emission maximum
443 nm
Optimal laser line
355
Optimal filter
450/50

BFP has an excitation maximum at 374 nm and an emission maximum at 443 nm, a Stokes shift of 69 nm. It is optimally excited by the 355 laser line.

Frequently asked questions

What is BFP?
BFP is a blue fluorescent protein derived from Aequorea victoria with an excitation peak at 374 nm and an emission peak at 443 nm.
What laser excites BFP?
BFP is optimally excited by the 355 laser line. Its excitation maximum is 374 nm.
What filter should I use to detect BFP?
A 450/50 filter captures the peak of the BFP emission near 443 nm with minimal spillover.