mKeima
mKeima (Ex-Max 440 nm/Em-Max 621 nm)
<html>mKeima is a monomric, pH-sensitive red fluorescent protein with excitation and emission maximums of 440 nm and 620 nm within the range of pH6-8, respectively. Below pH 6, the excitation max shifts to 486 nm with the same emission peak at 620 nm. mKeima was first derived from the stony coral, <i>Montipora sp 20.</i></html>
Excitation & emission spectra
Key facts
- Excitation maximum
- 440 nm
- Emission maximum
- 621 nm
- Optimal laser line
- 405
- Optimal filter
- 630/69
- Extinction coefficient
- 14400
- Quantum yield
- 0.24
mKeima has an excitation maximum at 440 nm and an emission maximum at 621 nm, a Stokes shift of 181 nm. Its extinction coefficient is 14400 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is mKeima?
- <html>mKeima is a monomric, pH-sensitive red fluorescent protein with excitation and emission maximums of 440 nm and 620 nm within the range of pH6-8, respectively. Below pH 6, the excitation max shifts to 486 nm with the same emission peak at 620 nm. mKeima was first derived from the stony coral, <i>Montipora sp 20.</i></html>
- What laser excites mKeima?
- mKeima is optimally excited by the 405 laser line. Its excitation maximum is 440 nm.
- What filter should I use to detect mKeima?
- A 630/69 filter captures the peak of the mKeima emission near 621 nm with minimal spillover.