Brilliant Ultraviolet 563
BUV563 (Ex-Max 348 nm/Em-Max 563 nm)
Brilliant™ UltraViolet 563 (BUV563) is a green-emitting tandem fluorophore that combines BD Horizon® BUV395 and an acceptor dye. It can be excited by the 355 nm UV laser and collected using a 585/15 bandpass filter. BUV563 has an excitation peak at 348 nm and an emission peak at 563 nm This dye is part of the Brilliant™ UltraViolet dye family, developed and sold by BD Biosciences. This family of dyes uses BUV395 as the donor so that all dyes in the family can be excited by the 355 nm UV laser. Depending on the acceptor, the dyes in this family have different relative brightness ratings.
Excitation & emission spectra
Key facts
- Excitation maximum
- 348 nm
- Emission maximum
- 563 nm
- Optimal laser line
- 355
- Optimal filter
- 585/42
- Relative brightness
- 3 of 5
- Commercial antibodies
- 801 from 2 suppliers
Brilliant Ultraviolet 563 has an excitation maximum at 348 nm and an emission maximum at 563 nm, a Stokes shift of 215 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is Brilliant Ultraviolet 563?
- Brilliant™ UltraViolet 563 (BUV563) is a green-emitting tandem fluorophore that combines BD Horizon® BUV395 and an acceptor dye. It can be excited by the 355 nm UV laser and collected using a 585/15 bandpass filter. BUV563 has an excitation peak at 348 nm and an emission peak at 563 nm This dye is part of the Brilliant™ UltraViolet dye family, developed and sold by BD Biosciences. This family of dyes uses BUV395 as the donor so that all dyes in the family can be excited by the 355 nm UV laser. Depending on the acceptor, the dyes in this family have different relative brightness ratings.
- What laser excites Brilliant Ultraviolet 563?
- Brilliant Ultraviolet 563 is optimally excited by the 355 laser line. Its excitation maximum is 348 nm.
- What filter should I use to detect Brilliant Ultraviolet 563?
- A 585/42 filter captures the peak of the Brilliant Ultraviolet 563 emission near 563 nm with minimal spillover.
- How bright is Brilliant Ultraviolet 563?
- Brilliant Ultraviolet 563 has a relative brightness of 3 out of 5.
- How many antibodies are available conjugated to Brilliant Ultraviolet 563?
- FluoroFinder tracks 801 commercially available Brilliant Ultraviolet 563 conjugates, searchable by target, clone, host and application.