iRFP
iRFP (Ex-Max 690 nm/Em-Max 713 nm)
iRFP is a dimeric fluorescent protein derived from the proteobacteria, Rhodopseudomonas palustris. It has an excitation peak at 690 nm and an emission peak at 713 nm. It requires the cofactor biliverdin for fluorescence.
Excitation & emission spectra
Key facts
- Excitation maximum
- 690 nm
- Emission maximum
- 713 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
- Extinction coefficient
- 105000
- Quantum yield
- 0.06
- Molecular weight
- 34600
iRFP has an excitation maximum at 690 nm and an emission maximum at 713 nm, a Stokes shift of 23 nm. Its extinction coefficient is 105000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is iRFP?
- iRFP is a dimeric fluorescent protein derived from the proteobacteria, Rhodopseudomonas palustris. It has an excitation peak at 690 nm and an emission peak at 713 nm. It requires the cofactor biliverdin for fluorescence.
- What laser excites iRFP?
- iRFP is optimally excited by the 628-640 laser line. Its excitation maximum is 690 nm.
- What filter should I use to detect iRFP?
- A 695/40 filter captures the peak of the iRFP emission near 713 nm with minimal spillover.