Nile Blue
Nile Blue (Ex-Max 628 nm/Em-Max 660 nm)
Nile Blue A is a chromogenic stain and fluorescent soluble cationic dye that stains cell membranes and can be sensitive to pH. Due to its solubility in aqueous medium, Nile Blue A can be easier to apply to histological samples for Fluorescence Microscopy. Spectrally it is also compatible with flow cytometry. Nile Blue is excited at 633 nm and emits at 660 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 628 nm
- Emission maximum
- 660 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Commercial antibodies
- 2 from 1 supplier
Nile Blue has an excitation maximum at 628 nm and an emission maximum at 660 nm, a Stokes shift of 32 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Nile Blue?
- Nile Blue A is a chromogenic stain and fluorescent soluble cationic dye that stains cell membranes and can be sensitive to pH. Due to its solubility in aqueous medium, Nile Blue A can be easier to apply to histological samples for Fluorescence Microscopy. Spectrally it is also compatible with flow cytometry. Nile Blue is excited at 633 nm and emits at 660 nm.
- What laser excites Nile Blue?
- Nile Blue is optimally excited by the 628-640 laser line. Its excitation maximum is 628 nm.
- What filter should I use to detect Nile Blue?
- A 660/20 filter captures the peak of the Nile Blue emission near 660 nm with minimal spillover.
- How many antibodies are available conjugated to Nile Blue?
- FluoroFinder tracks 2 commercially available Nile Blue conjugates, searchable by target, clone, host and application.