mBBR
mBBR (Ex-Max 390 nm/Em-Max 490 nm)
Monobromobimane (mBBR) is a cell permeant reagent that is fluorogenic upon conjugation to thiol moeities and thus can be used for detection and quantitation of glutathione, mercaptopurine, peptide and plasma thiols. It has an excitation peak at 390 nm and an emission peak at 490 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 390 nm
- Emission maximum
- 490 nm
- Optimal laser line
- 405
- Optimal filter
- 515/30
- Commercial antibodies
- 2 from 2 suppliers
mBBR has an excitation maximum at 390 nm and an emission maximum at 490 nm, a Stokes shift of 100 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is mBBR?
- Monobromobimane (mBBR) is a cell permeant reagent that is fluorogenic upon conjugation to thiol moeities and thus can be used for detection and quantitation of glutathione, mercaptopurine, peptide and plasma thiols. It has an excitation peak at 390 nm and an emission peak at 490 nm.
- What laser excites mBBR?
- mBBR is optimally excited by the 405 laser line. Its excitation maximum is 390 nm.
- What filter should I use to detect mBBR?
- A 515/30 filter captures the peak of the mBBR emission near 490 nm with minimal spillover.
- How many antibodies are available conjugated to mBBR?
- FluoroFinder tracks 2 commercially available mBBR conjugates, searchable by target, clone, host and application.