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Ghost Dye UV 450

Ghost Dye™ UV 450 (Ex-Max 342 nm/Em-Max 432 nm)

Ghost Dye™ UV 450 from Cytek is an amine-reactive viability dye used in flow cytometry when it will be necessary to fix and/or permeabilize the cells after staining. This reagent has an excitation peak at 342 nm and an emission peak at 432 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
342 nm
Emission maximum
432 nm
Optimal laser line
355
Optimal filter
450/50
Commercial antibodies
3 from 2 suppliers

Ghost Dye UV 450 has an excitation maximum at 342 nm and an emission maximum at 432 nm, a Stokes shift of 90 nm. It is optimally excited by the 355 laser line.

Frequently asked questions

What is Ghost Dye UV 450?
Ghost Dye™ UV 450 from Cytek is an amine-reactive viability dye used in flow cytometry when it will be necessary to fix and/or permeabilize the cells after staining. This reagent has an excitation peak at 342 nm and an emission peak at 432 nm.
What laser excites Ghost Dye UV 450?
Ghost Dye UV 450 is optimally excited by the 355 laser line. Its excitation maximum is 342 nm.
What filter should I use to detect Ghost Dye UV 450?
A 450/50 filter captures the peak of the Ghost Dye UV 450 emission near 432 nm with minimal spillover.
How many antibodies are available conjugated to Ghost Dye UV 450?
FluoroFinder tracks 3 commercially available Ghost Dye UV 450 conjugates, searchable by target, clone, host and application.