BODIPY TR
BODIPY TR (Ex-Max 585 nm/Em-Max 620 nm)
BODIPY TR is used as a lipophilic probe for polar and neutral lipids typically used to detect oil and nonpolar lipid droplets in live or fixed cells. It has an excitation peak at 585 nm and an emission peak at 620 nm. The succinimidyl ester form of this dye can be used to conjugate hydrophobic proteins or lipids.
Excitation & emission spectra
Key facts
- Excitation maximum
- 585 nm
- Emission maximum
- 620 nm
- Optimal laser line
- 561
- Optimal filter
- 610/20
- Molecular weight
- 634.5
BODIPY TR has an excitation maximum at 585 nm and an emission maximum at 620 nm, a Stokes shift of 35 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is BODIPY TR?
- BODIPY TR is used as a lipophilic probe for polar and neutral lipids typically used to detect oil and nonpolar lipid droplets in live or fixed cells. It has an excitation peak at 585 nm and an emission peak at 620 nm. The succinimidyl ester form of this dye can be used to conjugate hydrophobic proteins or lipids.
- What laser excites BODIPY TR?
- BODIPY TR is optimally excited by the 561 laser line. Its excitation maximum is 585 nm.
- What filter should I use to detect BODIPY TR?
- A 610/20 filter captures the peak of the BODIPY TR emission near 620 nm with minimal spillover.