DiD
DiD (Ex-Max 645 nm/Em-Max 668 nm)
DiD, also known as DiIC18(5), is a lipophilic carbocyanine dye often used in fluorescence microscopy to label the cell surface, trace neuronal axons or, upon fixation, any lipid bound compartment. This probe has an excitation peak at 645 nm and an emission peak at 668 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 645 nm
- Emission maximum
- 668 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Extinction coefficient
- 193000
- Molecular weight
- 1052.1
- Commercial antibodies
- 3 from 1 supplier
DiD has an excitation maximum at 645 nm and an emission maximum at 668 nm, a Stokes shift of 23 nm. Its extinction coefficient is 193000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is DiD?
- DiD, also known as DiIC18(5), is a lipophilic carbocyanine dye often used in fluorescence microscopy to label the cell surface, trace neuronal axons or, upon fixation, any lipid bound compartment. This probe has an excitation peak at 645 nm and an emission peak at 668 nm.
- What laser excites DiD?
- DiD is optimally excited by the 628-640 laser line. Its excitation maximum is 645 nm.
- What filter should I use to detect DiD?
- A 660/20 filter captures the peak of the DiD emission near 668 nm with minimal spillover.
- How many antibodies are available conjugated to DiD?
- FluoroFinder tracks 3 commercially available DiD conjugates, searchable by target, clone, host and application.