Alexa Fluor 568
Alexa Fluor™ 568 (Ex-Max 578 nm/Em-Max 603 nm)
Alexa Fluor™ 568 (AF568, Alexa 568) has an excitation peak at 578 nm and an emission peak at 603 nm, and is spectrally similar to Rhodamine B. Alexa 568 is commonly used fluorescence and super-resolution microscopy. Many flow cytometers are equipped with 561nm lasers, so while Alexa 568 can be used for flow cytometry, PE or NovaFluor Yellow 570 are more commonly used due to their brightness and spectral characteristics.
Excitation & emission spectra
Key facts
- Excitation maximum
- 578 nm
- Emission maximum
- 603 nm
- Optimal laser line
- 561
- Relative brightness
- 2 of 5
- Extinction coefficient
- 91000
- Quantum yield
- 0.69
- Molecular weight
- 792
- Commercial antibodies
- 552 from 9 suppliers
Alexa Fluor 568 has an excitation maximum at 578 nm and an emission maximum at 603 nm, a Stokes shift of 25 nm. Its extinction coefficient is 91000 M⁻¹cm⁻¹. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is Alexa Fluor 568?
- Alexa Fluor™ 568 (AF568, Alexa 568) has an excitation peak at 578 nm and an emission peak at 603 nm, and is spectrally similar to Rhodamine B. Alexa 568 is commonly used fluorescence and super-resolution microscopy. Many flow cytometers are equipped with 561nm lasers, so while Alexa 568 can be used for flow cytometry, PE or NovaFluor Yellow 570 are more commonly used due to their brightness and spectral characteristics.
- What laser excites Alexa Fluor 568?
- Alexa Fluor 568 is optimally excited by the 561 laser line. Its excitation maximum is 578 nm.
- How bright is Alexa Fluor 568?
- Alexa Fluor 568 has a relative brightness of 2 out of 5 (quantum yield 0.69).
- How many antibodies are available conjugated to Alexa Fluor 568?
- FluoroFinder tracks 552 commercially available Alexa Fluor 568 conjugates, searchable by target, clone, host and application.