What laser excites MitoTracker Orange?

MitoTracker Orange is optimally excited by the 532 laser line. Its excitation maximum is 550 nm.

What filter should I use to detect MitoTracker Orange?

A 585/42 filter captures the peak of the MitoTracker Orange emission near 573 nm with minimal spillover.

How many antibodies are available conjugated to MitoTracker Orange?

FluoroFinder tracks 2 commercially available MitoTracker Orange conjugates, searchable by target, clone, host and application.

MitoTracker Orange

MitoTracker Orange (Ex-Max 550 nm/Em-Max 573 nm)

MitoTracker Orange CMTMRos from Thermo Fisher Scientific is a potential-dependent mitochondrial localization probe with an excitation peak at 550 nm and an emission peak at 573 nm. It is recommended for fluorescence microscopy and live-cell imaging. It is retained with fixation using paraformaldehyde. This probe is also available in a reduced version that can aid in reducing background signal. The reduced version, MitoTracker Orange CM-H2TMRos, becomes more intensely fluorescent when it is oxidzed at the mitochondrial membrane.

Excitation & emission spectra

Key facts

Excitation maximum: 550 nm
Emission maximum: 573 nm
Optimal laser line: 532
Optimal filter: 585/42
Commercial antibodies: 2 from 1 supplier

MitoTracker Orange has an excitation maximum at 550 nm and an emission maximum at 573 nm, a Stokes shift of 23 nm. It is optimally excited by the 532 laser line.

Frequently asked questions

What is MitoTracker Orange?: MitoTracker Orange CMTMRos from Thermo Fisher Scientific is a potential-dependent mitochondrial localization probe with an excitation peak at 550 nm and an emission peak at 573 nm. It is recommended for fluorescence microscopy and live-cell imaging. It is retained with fixation using paraformaldehyde. This probe is also available in a reduced version that can aid in reducing background signal. The reduced version, MitoTracker Orange CM-H2TMRos, becomes more intensely fluorescent when it is oxidzed at the mitochondrial membrane.
What laser excites MitoTracker Orange?: MitoTracker Orange is optimally excited by the 532 laser line. Its excitation maximum is 550 nm.
What filter should I use to detect MitoTracker Orange?: A 585/42 filter captures the peak of the MitoTracker Orange emission near 573 nm with minimal spillover.
How many antibodies are available conjugated to MitoTracker Orange?: FluoroFinder tracks 2 commercially available MitoTracker Orange conjugates, searchable by target, clone, host and application.