BODIPY FL
BODIPY FL (Ex-Max 500 nm/Em-Max 509 nm)
BODIPY FL is used as a lipophilic probe for polar and neutral lipids typically used to detect oil and nonpolar lipid droplets in live or fixed cells. It has an excitation peak at 500 nm and an emission peak at 509 nm. The succinimidyl ester form of this dye can be used to conjugate hydrophobic proteins or lipids.
Excitation & emission spectra
Key facts
- Excitation maximum
- 500 nm
- Emission maximum
- 509 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
BODIPY FL has an excitation maximum at 500 nm and an emission maximum at 509 nm, a Stokes shift of 9 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is BODIPY FL?
- BODIPY FL is used as a lipophilic probe for polar and neutral lipids typically used to detect oil and nonpolar lipid droplets in live or fixed cells. It has an excitation peak at 500 nm and an emission peak at 509 nm. The succinimidyl ester form of this dye can be used to conjugate hydrophobic proteins or lipids.
- What laser excites BODIPY FL?
- BODIPY FL is optimally excited by the 488 laser line. Its excitation maximum is 500 nm.
- What filter should I use to detect BODIPY FL?
- A 515/30 filter captures the peak of the BODIPY FL emission near 509 nm with minimal spillover.