eFluor 514 Calcium Sensor
eFluor™ 514 Calcium Sensor (Ex-Max 490 nm/Em-Max 514 nm)
eFluor™ 514 (eF514) Calcium Sensor is a green-emitting calcium sensing dye that can be excited by the 488 nm blue laser and captured with a 525/50 bandpass filter. eF514 has an excitation peak at 490 nm and an emission peak at 514 nm, and is very similar spectrally and functionally to Fluo-3 AM and Fluo-4 AM, which are also calcium sensing dyes. This is a membrane-permeable calcium sensing dye that is used to monitor changes in calcium concentrations within the cell. eF514 Calcium Sensor can be used with fluorescence microscopy and microplate assay applications. This dye is part of the larger eFluor™ dye family, developed by eBioscience which is now part of ThermoFisher Scientific.
Excitation & emission spectra
Key facts
- Excitation maximum
- 490 nm
- Emission maximum
- 514 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Molecular weight
- 1100
eFluor 514 Calcium Sensor has an excitation maximum at 490 nm and an emission maximum at 514 nm, a Stokes shift of 24 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is eFluor 514 Calcium Sensor?
- eFluor™ 514 (eF514) Calcium Sensor is a green-emitting calcium sensing dye that can be excited by the 488 nm blue laser and captured with a 525/50 bandpass filter. eF514 has an excitation peak at 490 nm and an emission peak at 514 nm, and is very similar spectrally and functionally to Fluo-3 AM and Fluo-4 AM, which are also calcium sensing dyes. This is a membrane-permeable calcium sensing dye that is used to monitor changes in calcium concentrations within the cell. eF514 Calcium Sensor can be used with fluorescence microscopy and microplate assay applications. This dye is part of the larger eFluor™ dye family, developed by eBioscience which is now part of ThermoFisher Scientific.
- What laser excites eFluor 514 Calcium Sensor?
- eFluor 514 Calcium Sensor is optimally excited by the 488 laser line. Its excitation maximum is 490 nm.
- What filter should I use to detect eFluor 514 Calcium Sensor?
- A 525/50 filter captures the peak of the eFluor 514 Calcium Sensor emission near 514 nm with minimal spillover.