ATTO 550
ATTO 550 (Ex-Max 554 nm/Em-Max 576 nm)
ATTO 550 is from ATTO-Tec Gmbh has an excitation peak at 554 nm and an emission peak at 576 nm. ATTO 550 is a novel fluorescent label related to the well-known dyes Rhodamine 6G and Rhodamine B. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. The dye is moderately hydrophilic. ATTO 550 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. As supplied ATTO 550 consists of three isomers with practically identical absorption and fluorescence.
Excitation & emission spectra
Key facts
- Excitation maximum
- 554 nm
- Emission maximum
- 576 nm
- Optimal laser line
- 532
- Optimal filter
- 585/42
- Relative brightness
- 2 of 5
- Extinction coefficient
- 120000
- Quantum yield
- 0.8
- Fluorescence lifetime
- 3.6 ns
- Molecular weight
- 719
- Commercial antibodies
- 46 from 6 suppliers
ATTO 550 has an excitation maximum at 554 nm and an emission maximum at 576 nm, a Stokes shift of 22 nm. Its extinction coefficient is 120000 M⁻¹cm⁻¹. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is ATTO 550?
- ATTO 550 is from ATTO-Tec Gmbh has an excitation peak at 554 nm and an emission peak at 576 nm. ATTO 550 is a novel fluorescent label related to the well-known dyes Rhodamine 6G and Rhodamine B. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. The dye is moderately hydrophilic. ATTO 550 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. As supplied ATTO 550 consists of three isomers with practically identical absorption and fluorescence.
- What laser excites ATTO 550?
- ATTO 550 is optimally excited by the 532 laser line. Its excitation maximum is 554 nm.
- What filter should I use to detect ATTO 550?
- A 585/42 filter captures the peak of the ATTO 550 emission near 576 nm with minimal spillover.
- How bright is ATTO 550?
- ATTO 550 has a relative brightness of 2 out of 5 (quantum yield 0.8).
- How many antibodies are available conjugated to ATTO 550?
- FluoroFinder tracks 46 commercially available ATTO 550 conjugates, searchable by target, clone, host and application.