Fura Red AM (Ca2+free)
Fura Red AM (Ca2+free) (Ex-Max 470 nm/Em-Max 660 nm)
Fura Red, AM is an intracellular calcium indicator. When Fura Red is hydrolyzed intracellularly and binds cytoplasmic Ca2+, the excitation efficiency of the probe at 405 and 488nm decreases which diminishes the emission of the probe at ~660nm. It is commonly used with Fluo-4, AM which exhibits increased intensity when bound to ratiometrically assess calcium concentration at 488nm excitation.
Excitation & emission spectra
Key facts
- Excitation maximum
- 488 nm
- Emission maximum
- 660 nm
- Optimal laser line
- 488
- Optimal filter
- 660/20
- Commercial antibodies
- 1 from 1 supplier
Fura Red AM (Ca2+free) has an excitation maximum at 488 nm and an emission maximum at 660 nm, a Stokes shift of 172 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Fura Red AM (Ca2+free)?
- Fura Red, AM is an intracellular calcium indicator. When Fura Red is hydrolyzed intracellularly and binds cytoplasmic Ca2+, the excitation efficiency of the probe at 405 and 488nm decreases which diminishes the emission of the probe at ~660nm. It is commonly used with Fluo-4, AM which exhibits increased intensity when bound to ratiometrically assess calcium concentration at 488nm excitation.
- What laser excites Fura Red AM (Ca2+free)?
- Fura Red AM (Ca2+free) is optimally excited by the 488 laser line. Its excitation maximum is 488 nm.
- What filter should I use to detect Fura Red AM (Ca2+free)?
- A 660/20 filter captures the peak of the Fura Red AM (Ca2+free) emission near 660 nm with minimal spillover.
- How many antibodies are available conjugated to Fura Red AM (Ca2+free)?
- FluoroFinder tracks 1 commercially available Fura Red AM (Ca2+free) conjugates, searchable by target, clone, host and application.