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DyLight 800

DyLight™ 800 (Ex-Max 777 nm/Em-Max 794 nm)

DyLight™ 800 ihas an excitation peak at 777 nm and an emission peak at 794 nm and is spectrally similar to IRDye® 800. DyLight™ 800 is most commonly used in fluorescence microscopy and in vivo imaging applications.

Excitation & emission spectra

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Key facts

Excitation maximum
777 nm
Emission maximum
794 nm
Optimal laser line
785
Optimal filter
780/60
Extinction coefficient
270000
Commercial antibodies
126 from 11 suppliers

DyLight 800 has an excitation maximum at 777 nm and an emission maximum at 794 nm, a Stokes shift of 17 nm. Its extinction coefficient is 270000 M⁻¹cm⁻¹. It is optimally excited by the 785 laser line.

Frequently asked questions

What is DyLight 800?
DyLight™ 800 ihas an excitation peak at 777 nm and an emission peak at 794 nm and is spectrally similar to IRDye® 800. DyLight™ 800 is most commonly used in fluorescence microscopy and in vivo imaging applications.
What laser excites DyLight 800?
DyLight 800 is optimally excited by the 785 laser line. Its excitation maximum is 777 nm.
What filter should I use to detect DyLight 800?
A 780/60 filter captures the peak of the DyLight 800 emission near 794 nm with minimal spillover.
How many antibodies are available conjugated to DyLight 800?
FluoroFinder tracks 126 commercially available DyLight 800 conjugates, searchable by target, clone, host and application.