Rhodamine 110
Rhodamine 110 (Ex-Max 497 nm/Em-Max 520 nm)
Rhodamine 110 is a generic fluorophore that has an excitation peak at 497 nm and an emission peak at 520 nm. Spectrally, its similar to FITC and Alexa Fluor™ 488.
Excitation & emission spectra
Key facts
- Excitation maximum
- 497 nm
- Emission maximum
- 520 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Molecular weight
- 366.8
Rhodamine 110 has an excitation maximum at 497 nm and an emission maximum at 520 nm, a Stokes shift of 23 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Rhodamine 110?
- Rhodamine 110 is a generic fluorophore that has an excitation peak at 497 nm and an emission peak at 520 nm. Spectrally, its similar to FITC and Alexa Fluor™ 488.
- What laser excites Rhodamine 110?
- Rhodamine 110 is optimally excited by the 488 laser line. Its excitation maximum is 497 nm.
- What filter should I use to detect Rhodamine 110?
- A 525/50 filter captures the peak of the Rhodamine 110 emission near 520 nm with minimal spillover.