DyLight 680
DyLight™ 680 (Ex-Max 692 nm/Em-Max 712 nm)
DyLight™ 680 has an excitation peak at 692 nm and an emission peak at 712 nm, and is spectrally similar to Alexa Fluor™ 680 and Cy5.5. DyLight™ 680 is most commonly used in flow cytometery and fluorescence microscopy applications.
Excitation & emission spectra
Key facts
- Excitation maximum
- 692 nm
- Emission maximum
- 712 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
- Relative brightness
- 1 of 5
- Extinction coefficient
- 140000
- Commercial antibodies
- 21.6k from 14 suppliers
DyLight 680 has an excitation maximum at 692 nm and an emission maximum at 712 nm, a Stokes shift of 20 nm. Its extinction coefficient is 140000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is DyLight 680?
- DyLight™ 680 has an excitation peak at 692 nm and an emission peak at 712 nm, and is spectrally similar to Alexa Fluor™ 680 and Cy5.5. DyLight™ 680 is most commonly used in flow cytometery and fluorescence microscopy applications.
- What laser excites DyLight 680?
- DyLight 680 is optimally excited by the 628-640 laser line. Its excitation maximum is 692 nm.
- What filter should I use to detect DyLight 680?
- A 695/40 filter captures the peak of the DyLight 680 emission near 712 nm with minimal spillover.
- How bright is DyLight 680?
- DyLight 680 has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to DyLight 680?
- FluoroFinder tracks 21610 commercially available DyLight 680 conjugates, searchable by target, clone, host and application.