CF750
CF®750 (Ex-Max 755 nm/Em-Max 777 nm)
CF®750 from Biotium is a fluorophore with an excitation peak at 755 nm and an emission peak at 777 nm. CF®750 has been specifically validated in super-resolution imaging by STORM. It is spectrally similar to Alexa Fluor™ 750 and Cy7.
Excitation & emission spectra
Key facts
- Excitation maximum
- 755 nm
- Emission maximum
- 777 nm
- Optimal laser line
- 730-750
- Optimal filter
- 780/60
- Relative brightness
- 1 of 5
- Extinction coefficient
- 250000
- Molecular weight
- 3009
- Commercial antibodies
- 10 from 1 supplier
CF750 has an excitation maximum at 755 nm and an emission maximum at 777 nm, a Stokes shift of 22 nm. Its extinction coefficient is 250000 M⁻¹cm⁻¹. It is optimally excited by the 730-750 laser line.
Frequently asked questions
- What is CF750?
- CF®750 from Biotium is a fluorophore with an excitation peak at 755 nm and an emission peak at 777 nm. CF®750 has been specifically validated in super-resolution imaging by STORM. It is spectrally similar to Alexa Fluor™ 750 and Cy7.
- What laser excites CF750?
- CF750 is optimally excited by the 730-750 laser line. Its excitation maximum is 755 nm.
- What filter should I use to detect CF750?
- A 780/60 filter captures the peak of the CF750 emission near 777 nm with minimal spillover.
- How bright is CF750?
- CF750 has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to CF750?
- FluoroFinder tracks 10 commercially available CF750 conjugates, searchable by target, clone, host and application.