DyLight 594
DyLight™ 594 (Ex-Max 593 nm/Em-Max 618 nm)
DyLight™ 594 has an excitation peak at 593 nm and an emission peak at 618 nm and is spectrally similar to Alexa Fluor™ 594 and Texas Red. DyLight™ 594 is most commonly used in flow cytometery and fluorescence microscopy applications.
Excitation & emission spectra
Key facts
- Excitation maximum
- 593 nm
- Emission maximum
- 618 nm
- Optimal laser line
- 594
- Optimal filter
- 630/69
- Relative brightness
- 2 of 5
- Extinction coefficient
- 80000
- Commercial antibodies
- 23k from 12 suppliers
DyLight 594 has an excitation maximum at 593 nm and an emission maximum at 618 nm, a Stokes shift of 25 nm. Its extinction coefficient is 80000 M⁻¹cm⁻¹. It is optimally excited by the 594 laser line.
Frequently asked questions
- What is DyLight 594?
- DyLight™ 594 has an excitation peak at 593 nm and an emission peak at 618 nm and is spectrally similar to Alexa Fluor™ 594 and Texas Red. DyLight™ 594 is most commonly used in flow cytometery and fluorescence microscopy applications.
- What laser excites DyLight 594?
- DyLight 594 is optimally excited by the 594 laser line. Its excitation maximum is 593 nm.
- What filter should I use to detect DyLight 594?
- A 630/69 filter captures the peak of the DyLight 594 emission near 618 nm with minimal spillover.
- How bright is DyLight 594?
- DyLight 594 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to DyLight 594?
- FluoroFinder tracks 22978 commercially available DyLight 594 conjugates, searchable by target, clone, host and application.